Analyses of cartilage superficial layer using immunohistochemical staining
نویسندگان
چکیده
منابع مشابه
Immunohistochemical demonstration of fibronectin in the most superficial layer of normal rabbit articular cartilage.
OBJECTIVE To locate fibronectin ultrastructurally in the most superficial layer of normal articular cartilage of rabbits, in order to clarify its role in joint physiology. METHODS Articular cartilage was obtained from the femoral condyle of seven normal adult rabbits and prepared by a method that included tannic acid fixation. Polyclonal antibodies against rabbit fibronectin were used in an i...
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We studied the most superficial layer of macroscopically normal articular cartilage obtained from human femoral heads, using polarising microscopy and SEM. The most superficial layer, 4 to 8 microns thick, was acellular consisting of collagen fibrils. This layer could be peeled away as a thin film, with no broken collagen fibrils on its inferior surface or on the surface of subjacent cartilage ...
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By studying changes in the knee joint, Bennett, Waine, and Bauer (1942) were able to trace the development of osteo-arthritis. They showed that the condition initially affects the articular cartilage, a conclusion subsequently supported by Collins (1949). Osteo-arthritic fibrillation is the hallmark of the cartilage lesion. The fibrillated cartilage becomes soft and moist in texture. Chemical a...
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Despite numerous attempts to develop and evalute specsc methods for the histochemical demonstration of mucopolysaccharides, definitive information is not readily available. Thls is in spite of the notable contributions of Hale ('46); Steedman ('50); Gomori ('50); Kramer and Windrum ('54); Mowry ('56, '58); Spicer ('60); Belanger and Migicovsky ('61) and Goldstein ('62) to mention only a few. Wi...
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OBJECTIVE The purpose of this study is to investigate molecular subtyping and its implications on metaplastic carcinoma according to surrogate immunohistochemical (IHC) staining. METHODS Following tissue microarray analysis of 34 cases of metaplastic carcinoma, IHC staining for cytokeratin (CK) 5/6, epidermal growth factor receptor (EGFR), claudin-3, claudin-4, claudin-7, E-cadherin, STAT-1, ...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2012
ISSN: 1063-4584
DOI: 10.1016/j.joca.2012.02.161